Professor Bengt Carlsson reached out to the Testa Center in February 2021 with an ask. “Would it be possible to have students in the Testa Center using bioreactors to learn PID control?” he asked, yes, certainly we said.
After two short visits to the laboratory and Bengt and the Testa Center staff prepared the experiment instruction for the students. And when the time came, in October that year, the first students arrived and successfully performed the lab. The students were thrilled to use real-world, industry-grade, bioreactors. And when the PID parameters had been fully optimized, then everyone, including Bengt, went home with new knowledge of hands-on control of bioreactors and big smiles on their faces.
“Normally we use a lot of simulation exercises … it’s not the real thing, and here they could really try the method on the real process”.
Bengt Carlsson, 2021.
Testa Center embraces academic work in life science. We love to work together with academia and other educational organizations to secure excellence in the life science sector. Would you like to know more, please read another testimonial or get in contact with us.
Introduction to filtration for application specialists by training and example.
The first part is filtration technology training on Pall products. The second part describes a large-scale ribosome purification project that ran in Testa Center. The third part consists of an introduction to the Testa Center. In the fourth and last part, the presenters answer questions from the audience.
Questions from the audience for the presenters to answer.
Thank you very much to everyone in the audience for listening and for all your questions. Thank you to all presenters for the well-prepared presentations which were all nicely delivered in this webinar.
The Protein Science Facility, Testa Center, and STUNS Life Science had a joint presentation on the 29th of November 2021. How can Karolinska Institutet utilize these support organizations and their resources for large-scale biomolecule production?! Speakers Tomas Nyman, Head of the Protein Science Facility (PSF) Karolinska Institutet / SciLifeLab. On PSF services. General agreement between …
Introduction to filtration for application specialists by training and example. Webinar content The first part is filtration technology training on Pall products. The second part describes a large-scale ribosome purification project that ran in Testa Center. The third part consists of an introduction to the Testa Center. In the fourth and last part, the presenters …
This webinar was held for Academia to learn what Testa Center can provide for them and their academic ambitions. The speakers An introduction to the Testa Center and academic collaboration – What’s in the Testa Center that benefits academic education and research? Exemplifying by describing the collaboration with Uppsala University, by Jesper Hedberg, Director at Testa …
During December 2020, final-year students from Uppsala university learned all about bioprocess optimization for cell growth and purification, and what life is like when you are employed at a bioprocess company.
All the students were from Uppsala University’s Master’s program in Molecular Biotechnology Engineering. This project is the main topic for their last course of the program named “Molecular Biotechnology for Production”. The students were asked by an industrial customer to carry out process development in order to deliver optimized processes for cell culturing of protein in E. coli. and purification using chromatography.
Working independently, the students enjoyed the opportunity and learned what it means to work many long hours with full responsibility for their own lab, processes and results. Karin Stensjö (Senior lecturer and associate professor at the Department of Chemistry in Ångström Laboratory at Uppsala University) who supervised the students was delighted that they were all fully engaged and eager to learn.
Testa Center made a series of interviews, which are summarized here.
Who was involved in the project?
The Downstream Team comprised Therese Koivula, Gustav Johnson and Louise Fitkin.
The Upstream Team comprised Jenny Hännestrand, Gabriel Chumpitaz Chavez, Anisha Khan and Hedvig Kindmark.
The course is ten weeks long and gives the student 15 hp (Swedish university credits) when completed. It is in addition to the Master’s Thesis to complete the five-year program.
What is your project assignment?
“We received an assignment to optimize a process protocol. It’s a “wing-to-wing” assignment and a “Do-It-Yourself” project where we own all the work – from learning how to carry out the process and the experimentation to presenting the results to the customer.”
What are you up to right now?
The Upstream Team is optimizing protein expression in E. coli using industrial bio reactors (Applikon ez2-controller parallel reactors).
Meanwhile, the Downstream Team first optimized the use of different salt fractions and will then start refining the process using different pH-concentrations on an ÄKTA pure 25 system from Cytiva.
When asked where most time was being spent, both groups spontaneously answered the same thing – “Learning!” They went on to explain, “Full ownership of the task at hand, the equipment, and experimenting without guidance makes us revisit many things we have learned in earlier courses. Not only that, but we also need to learn how to use and interpret instruments and results we’ve never seen before.”
Is this different from what you do in University?
“University lab exercises come with paper instructions and the result is often known. This project is open-ended in the sense that our results will be reviewed by a real industrial customer…” “… Another thing is the scale of the equipment in Testa Center. It is very rewarding when you get large-scale equipment working using method programming.”
“Usually, we share the university labs with others. Sharing means you have limited time and equipment to do your experiments. We were lucky to be able to run our project in Testa Center where we had both the time and resources to try things over until we got them right.”
How does this affect you when looking for a job?
“I think this project was a great experience. It has been very inspiring for my future career,” Jenny said.
“We have had a lot of fun in the Testa Center…” and ” …It has opened my eyes to the great fun of practical work in the lab,” Therese added.
What was the best part of being in Testa Center?
“Best was being able to do everything yourself, from preparing buffers to presenting the results. Another thing is that you get hands-on experience with modern equipment actually being used in industry – that’s super valuable!”