Scale up your bioprocess with less risk

Biological Production

Biological Production

Biological Production

Biological production at Testa Center enables startups, small and large companies, researchers, and academics to evaluate and verify bioprocesses in a pilot-scale environment before moving toward large scale or commercial manufacturing. Whether the goal is to develop a scale-up strategy, generate biological material for research and development, or demonstrate proof of concept for a new method, the facility provides the space for testing and validation. Projects are led by the client, who retains ownership of their intellectual property, while Testa Center staff provide guidance and technical support throughout the process.

The facility supports end-to-end bioprocessing activities through upstream and downstream operations, including cell cultivation, culture analytics, harvest, purification, purification analytics, and fluid handling. Applications may include recombinant proteins, monoclonal antibodies (mAbs), enzymes, viral vectors, nucleic acid therapeutics, vaccines, and other biologics.

Biological Production

Bioprocessing Techniques

Below are a few examples of the upstream and downstream processes which are compatible with our facility.

Cell Culturing

Supporting process development, scale-up, and production of cell cultures.​

Culturing Analytics

Allowing for monitoring and optimization of cell growth, viability, and process performance.

Harvest

Supporting cell separation and product recovery following cultivation.

Purification

Product capture and purification to achieve target purity and yield.

Purification Analytics

Product characterization and quality assessment throughout downstream processing.

Fluid Handeling

Supporting preparation, transfer, storage, and management of media, buffers, samples, and process fluids across the bioprocess.

Biological Production

Case studies

Wondering what kinds of biological projects you can do at Testa Center? Read through a few client projects to get an idea of what’s possible. Project areas range in focus from scale up & tech transfer to producing large quantities of biological materials.

Scale-up & tech transfer

Intervacc's Vaccine Production for Pigs

Intervacc AB

Streptococcus suis is affecting pig farmers globally, causing sepsis, meningitis, and arthritis in piglets. With Testa Center’s support, it took Intervacc AB 6 months to…

Intervacc's Vaccine Production for Pigs

Streptococcus suis is affecting pig farmers globally, causing sepsis, meningitis, and arthritis in piglets. With Testa Center’s support, it took Intervacc AB 6 months to optimise the production process for the first fusion protein. They succeeded in increasing the density of the bacteria in the fermenters almost 30-fold. After refining the purification steps, the protein’s purity exceeded 95%. From just one litre of bacterial culture, they can now produce enough protein to vaccinate a quarter of a million pigs. 

“When the time comes to transfer the vaccine to a GMP manufacturer, we will already have in-house knowledge about the production process. So, we’re expecting far fewer surprises and a much faster and more efficient hand-over to our GMP partners.” Andrew Waller, Chief Scientific Officer at Intervacc AB

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BioLamina's Parkinson's Solution

BioLamina

In 2018, as part of a greater collaboration with Novo Nordisk and Lund University, BioLamina came to Testa Center to scale up their production capacity for their MX/CTG Laminin 521 product, intended to support…

BioLamina's Parkinson's Solution

In 2018, as part of a greater collaboration with Novo Nordisk and Lund University, BioLamina came to Testa Center to scale up their production capacity for their MX/CTG Laminin 521 product, intended to support cell therapy products targeting patients with Parkinson’s disease.

“Now, we have designed a process that allows us to produce and process 40 liters of harvest multiple times in a month, if necessary,” explains Parinith Sudhakar, a former upstream scientist from BioLamina.

By the time they concluded their project, the team from BioLamina had acquired a new educational level in bioprocessing, including do-it-yourself and hands-on experience. The project also provided them with additional knowledge about laboratory design, which they used to build their own laboratory in an efficient way.

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Producing Materials

SLU's Yeast-based Oil Production

Sveriges lantbruksuniversitet

Prof. Volkmar Passoth and his team from the Department of Molecular Sciences at Swedish University of Agricultural Sciences came to Testa Center with two main objectives…

SLU's Yeast-based Oil Production

Prof. Volkmar Passoth and his team from the Department of Molecular Sciences at Swedish University of Agricultural Sciences came to Testa Center with two main objectives: 1) study the effects of scaling up production from laboratory to industrial scale, and 2) to produce sufficient amount of oil for further testing of advanced methods and applications regarding yeast-based oil production. They were able to successfully produce high amounts of lipid-rich biomass, which solved an existing bottleneck in the testing of various downstream techniques and applications

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Anna Rising Spider Silk

Sveriges lantbruksuniversitet

Spider silk cannot be harvested from its natural source on a large scale, because spiders are territorial and cannibalistic. An alternative approach is to…

Anna Rising Spider Silk

Spider silk cannot be harvested from its natural source on a large scale, because spiders are territorial and cannibalistic. An alternative approach is to manufacture an artificial replica using a biological production process. The Anna Rising research group has developed a mini-variant of spider silk proteins that can be obtained in very high yields with bacterial cell cultures. By means of a simple pH transition it is possible to convert soluble proteins into a solid fiber with a toughness that matches natural spider silk and very good mechanical properties, which can be spun using a biomimetic approach.

To address the challenge of upscaling the production and purification of spider silk proteins from the gram to the kg scale, Benjamin and Tomás planned and organized a 6-week project at Testa Center during 2023, together with their team of Viktoria, Frédéric, Urmi, Elisabeth, Lilian and Emilia.

“The lab at Testa Center comes with the advantage of high flexibility and high-end equipment combined with a world-class staff! Apart from the massive amounts of protein purified, the experience and knowledge that we have gained during our time at Testa Center is priceless!”

The next step in the development of this technology is to transition from making monofilaments into multifilaments and the manufacturing of silk yarns. By doing this work the research group hope to contribute to harmonizing the demand of our society for high-performance fibers with a reduced burden on the environment.

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Ready to start testing?

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Testa Center is an open and friendly testbed for biological production. Come to us and leverage the bioprocessing labs, equipment and expertise for the success of your project.

 

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